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. 2022 Sep 15;14(18):3812. doi: 10.3390/nu14183812

Figure 6.

Figure 6

GF blocks dermal fibroblastic apoptosis and inflammation caused by BPA. (A) Time-dependent response of expression of cleaved caspase-3, Bcl-2, and Bax in NHDFs exposed by BPA are shown. n = 3. * p ≤ 0.05 vs. 0 h. ROD, relative optical density. (B) The inhibitory effects of GF on expression of apoptosis-related proteins in BPA-treated NHDFs are shown. n = 4. * p ≤ 0.01 vs. Cont., # p ≤ 0.01 vs. BPA alone. (C) NHDFs were pretreated with Bay 11-7082 for 30 min prior to BPA exposure for 6 h. n = 4. * p ≤ 0.01 vs. Cont. # p ≤ 0.01 vs. BPA alone. (D) NHDFs were incubated with GF for 30 min prior to BPA exposure for 6 h. The apoptotic cell proportion stained by Annexin V/PI was analyzed by performing flow cytometry. n = 4. * p ≤ 0.01 vs. control. (E) The level of ATP production regulated by GF in BPA-treated NHDF for 6 h. * p ≤ 0.05 vs. Cont. # p ≤ 0.01 vs. BPA alone. n = 4. (F) Cells were treated with NAC, PD98059, and Bay11-7082 for 30 min prior to BPA exposure for 6 h. w is shown. * p ≤ 0.01 vs. Cont. # p ≤ 0.05 vs. BPA alone. n = 4. The green squares indicate the cells treated with GF, NAC, PD98059, or Bay 11-7082 alone.