Figure 6.
PAMAM NCs do not affect M2 macrophage viability under normoxic and hypoxic conditions. BMDM cell viability was determined by live/dead assays. The polarized M2 BMDMs were cultured in normoxic (21% O2) (a) or hypoxic (1% O2) (b) conditions and treated with corresponding PAMAM NCs (PG3 1/1, PG3 2/1, PG4 1/1 or PG4 2/1), cGAMP (25 µg/condition) or with media. After 24 h, cells were collected stained using a Live/Dead Viability Assay Kit to evaluate cell viability and analyzed by flow cytometry.