Table 2.
Results of HIV-1-DNA quantification and characterization in patient IciS-05.
| Time Point (Days) | Chimerism (%) | Ultrasensitive RNA Quantification (Copies/mL) | Patient Material | ddPCR (Copies/106 Cells) |
Gp120V3-Sequence (Dominant FPRs; Range, %) | |
|---|---|---|---|---|---|---|
| HIV-1 LTR | HIV-1 Pol | |||||
| Pre allo-HSCT | ||||||
| −20 | 15 | PBMCs | 1967 | 432 | 87.2, 89.7 (68.8–96.2) | |
| Tn | 1284 | 167 | 87.2, 89.7 (87.2–89.7) | |||
| Tcm | 3074 | 609 | 87.2, 89.7 (87.2–91.0) | |||
| Ttm | 5600 | 1622 | 87.2, 89.7 (68.0–89.7) | |||
| Tem | 6924 | 1886 | 87.2, 89.7 (68.8–89.7) | |||
| −19 | BM | 1135 | 167 | 87.2, 89.7(73.7–89.7) | ||
| Post allo-HSCT | ||||||
| +5 | 3 | PBMCs | 949 | ND | ||
| +16 | 57 | PBMCs | 278 | <21 | ||
| +36 | 100 | 0 | PBMCs | <5 | ND | |
| +54 | 95 | 8 | PBMCs | 19 | ND | |
| +61 | PBMCs | <20 | <20 | |||
| +65 | 85 | |||||
| +68 (died) | 8 | PBMCs | <7 | <22 | ||
| Post-mortem biopsies (one biopsy from same site is separated in two parts; 1,2) | ||||||
| +69 | Liver, biopsy 1; 2 | 54; trace | ND; <8 | No amplification | ||
| Lung left, biopsy 1; 2 | 36; 49 | ND; trace | 89.7, 87.2 (87.2–89.7) | |||
| Lung right, biopsy 1; 2 | 90; 32 | ND; trace | 89.7, 87.2 (71.4–92.2) | |||
| Spleen, biopsy 1; 2 | 43; 67 | ND; 34 | No amplification | |||
| Terminal Ileum, biopsy 1; 2 | 549; 81 | ND; 89 | 89.7, 87.2 (64.4–89.7) | |||
Allo-HSCT, allogeneic hematopoietic stem cell transplantation. BM, bone marrow. ddPCR, droplet digital PCR. FPR, false-positive-rate. LTR, long terminal repeat DNA sequence. ND, not done. NTC, no template control. PBMCs, peripheral blood mononuclear cells. pol, DNA polymerase DNA sequence. Tcm, central memory T-cell. Tem, effector memory T-cell. Tn, naive T-cell. Ttm, transitional memory T-cell.