Figure 5.

Effect of TLR-4 activation induced miR-125a on monocyte chemotaxis. CCR2-specific monocyte chemotaxis recorded by time-lapse microscopy was analysed using single-cell tracking. Control- or LPS-stimulated monocytes were incubated in a chemotaxis slide (ibidi). Arrows below the plot indicate the CCL2 concentration gradient: (A) centre of mass represents the average of all single-cell endpoints; (B) one representative chemotaxis plot of three independent experiments; (C–E) directional migration, velocity and accumulated distance in LPS-treated and control monocytes. * p < 0.05, ** p < 0.01.