Table 1.
RT-qLAMP primer sets used in this study.
| Virus and Primer Name | Primer Sequences (5′–3′) |
|---|---|
| ADV | |
| F3 | CTGCTCTCACAGATCACGG |
| B3 | CCCCGCCAAACATCTTGC |
| FIP | AACGTAGGGGCAGGTGCGG-CAGCATCGGAGGAGTCCA |
| BIP | CGTCCTATCGAGCCGCACTTT-CCCCAGCCTGTGTTATTGC |
| LF | CGTCAGTAATGGTCACTCGC |
| LB | AACATGTCCATCCTTATATCGCC |
| Flu A/H1N1 (Hemagglutinin gene set 1) | |
| F3 | TGAAGTTACTAATGCTACTGAGCTG |
| B3 | TCCCTCACTTTGGGTCTT |
| FIP | GACTTTGTTGGTCAGCACTAGTAGTAGATTTTAAAGGGAAAGAAGTCCTCG |
| BIP | ATCAGAATGCAGATGCATATGTTTTGCTATTTCCGGCTTGAACT |
| LF | CGATACCCCGTAAGTGGTAG |
| LB | TTTTGTGGGGACATCAAGATACAG |
| Flu A/H1N1 (Hemagglutinin gene set 2) | |
| F3 | AGCTAAGAGAGCAATT |
| B3 | TTTCCCTTTATCATTAATGTAGGATTTG |
| FIP | ACCTTTGTTCGAGTCATGATTGGTCTCAGTGTCATCATTTGAAAGGTTT |
| BIP | TAACGGCAGCATGTCCTCAGTATGAATTTCCTTTTTTAACTAGCCA |
| LF | CCATGAACTTGTCTTGGGGAATA |
| LB | GCTGGAGCAAAAAGCTTCTACA |
| Flu A/H3N2 (Hemagglutinin gene set 1) | |
| F3 | TGAGCTACATTCTATGTCTGG |
| B3 | GTGAGGACTGTCGCATAT |
| FIP | CGTTTGGTACTGCATGGTGCTTTTCGCTCAAAAAATTCCTGG |
| BIP | AGTGAAAACAATCACAAATGACCGCACCTATTGAGGAATTCTGAAC |
| LF | AGCGTCGCCGTGCTATTGT |
| LB | TGAAGTTACTAATGCTACTGAGTTGG |
| Flu A/H3N2 (Hemagglutinin gene set 2) | |
| F3 | CAAGAGAACCTTATGTGTCATG |
| B3 | AACTTGAGCTGGACCATG |
| FIP | ACTGTGTTATTTGAATGCACGTTGTCAAGTGTTATCAATTTGCCCT |
| BIP | CGTGATAGGACCCCTTATCGGACTATGCACACTTGCTTGG |
| LB | ATTGATGAATGAGTTGGGTGTTCCT |
| RSV A | |
| F3 | GATCTGCAATCGCCAGTG |
| B3 | TCTATCACAGTTTCAATGTTTGA |
| FIP | TGTGGATAGTAGAGCACTTTTGATTGCATTGCCGTATCCAAGG |
| BIP | CTAATGGAGTCAGTGTCTTAACCAGGCTTGTTAACAATAGGTAACAACT |
| LF | GTTCACTTCCCCTTCTAGGTGTA |
| RSV B | |
| F3 | CACCAGCTGTCAACAACC |
| B3 | GCATTTTTGATCTTGTTCACTT |
| FIP | TCGTTTCCTCTTCTTGCTTATTGATCAGAAGAGAAGCACCACA |
| BIP | ATTTCTGGGCTTCTTGTTAGGTGCTCCTTCAAGGTGTAGAACTT |
| LF | TCTGCAATAGCAAGTGGTATAGCT |
| Influenza B | |
| F3 | GGACATGAACAACAAAGATGC |
| B3 | GGCAACAAGTTTAGCAACAA |
| FIP | GGACAATACATTACGCATATCCCTTGATAAAGGAGGAAGTAAACACTCA |
| BIP | GGAACATTCCTCAAACACCCCAGCCTTCCACTCTGGTCAT |
| LF | GTCAAACGGAACTTCCCTTCTTTC |
| LB | GATACAAGTCCTTATCAACTCTGCA |
| SARS-CoV-2 (orf1ab gene) | |
| F3 | TGCAACTAATAAAGCCACG |
| B3 | CGTCTTTCTGTATGGTAGGATT |
| FIP | TCTGACTTCAGTACATCAAACGAATAAATACCTGGTGTATACGTTGTC |
| BIP | GACGCGCAGGGAATGGATAATTCCACTACTTCTTCAGAGACT |
| LF | TGTTTCAACTGGTTTTGTGCTCCA |
| LB | TCTTGCCTGCGAAGATCTAAAAC |
| SARS-CoV-2 (S gene) (S gene) |
|
| F3 | CTGACAAAGTTTTCAGATCCTCAG |
| B3 | AGTACCAAAAATCCAGCCTCTT |
| FIP | TCCCAGAGACATGTATAGCATGGAATCAACTCAGGACTTGTTCTTACC |
| BIP | TGGTACTAAGAGGTTTGATAACCCTGTTAGACTTCTCAGTGGAAGCA |
| LF | CCAAGTAACATTGGAAAAGAAA |
| LB | GTCCTACCATTTAATGATGGTGTTT |
ADV: adenovirus, F3: outer forward primer, B3: outer backward primer, FIP: forward inner primer, BIP: backward inner primer, LF: loop forward primer, LB: loop backward primer. RSV: respiratory syncytial virus, SARS-CoV-2: severe acute respiratory syndrome coronavirus 2.