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Western blot images of Formulations 1 and 2. (A): Formulation 1. Lane 1, 2: HPV16 VLPs in MPs (1.0 and 0.5 μg), Lane 3, 4, 5, 6, 7 and 8: blank MPs + HPV 16 VLPs in solution (1.0, 0.5, 0.25, 0.125, 0.06 and 0.03 μg), Lane 9: Blank MP. (B): Formulation 2 contained 20% CPD, and HPV16 VLP absorbed into alum and MPL. Lane 1, 2, 3, 4 and 5: VLPs + Blank MPs (2.0, 1.0, 0.5, 0.25 and 0.125 μg), Lane 6: Blank MP, Lane 7, 8 and 9: HPV16 VLPs + Alum + MPL MPs (2.0, 1.0 and 0.5 μg).
