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Parameters for binding of the CBDCelK and its deletion and mutation variants to ASC and BMCC calculated from adsorption data
| Protein | Capacitya (μmoles/g of cellulose); Krb (liters/g)
|
|
|---|---|---|
| ASC | BMCC | |
| CBDCelK | 17.1; 2.33 | 3.95; 9.87 |
| CBD-NCelK | 16.2; 2.67 | 3.86; 11.00 |
| D192A | 15.7; 1.84 | 3.68; 6.88 |
| Y136A | 3.46; 0.38 | 0.94; 1.06 |
| Y111A | 1.39; ND | |
Maximum amount of protein bound to 1 g of cellulose.
Relative equilibrium association constant (15).
