Figure 4.

Humoral immune responses after taRNA vaccination. (A): Graphic illustration of the vaccination scheme. For vaccination, mice received the respective RNAs on day 0 and day 28 intradermally. As negative control, mice received PBS. For the analysis of humoral immune responses, blood was collected on day 28 and day 56. (B): CHIKV-binding, (C): RRV-binding, and (D): MAYV-binding IgG antibodies in mouse sera. Endpoint titers were calculated and were set to 10 for samples that were below the cutoff. The RNA doses and combinations are indicated. The mean values ± SEM of each group (n = 5) are depicted. For statistical analysis, a two-way ANOVA with Dunnett’s multiple comparison post-test was performed (* p < 0.05; ** p < 0.01; *** p < 0.001).