Table 3.
Characteristics of the mutant positions in subcultures of M. tuberculosis H37Rv grown under different concentrations of 11.
| Position in Genome |
Ref | Mut | Gene | Amino Acid Change | PAM1 * | SIFT p ** | % of Mutant Reads in H37Rv Cultured with 11 (1.00 µM) |
% of Mutant Reads in H37Rv Cultured with 11 (2.00 µM) |
% of Mutant Reads in H37Rv Cultured with 11 (4.00 µM) | p Value for the Most Contrasting Pairs |
|---|---|---|---|---|---|---|---|---|---|---|
| 268,560 | A | T | Rv0224c | Phe23Tyr | 21 | 0.01 | 9.6 (13/136) | 15.8 (29/183) | 20.0 (25/125) | 0.02 |
| 411,895 | A | T | Rv0342 (iniA) | Gln353Leu | 6 | 0.02 | 12.7 (21/165) | 8.9 (16/180) | 23.8 (35/147) | 0.0004 |
| 1,305,250 | C | G | Rv1173 (fbiC) | Arg774Gly | 1 | 0.00 | 14.0 (18/129) | 12.8 (21/164) | 17.2 (22/128) | 0.3 |
| 1,783,849 | G | C | Rv1580c | Ala15Gly | 21 | 0.00 *** | 22.2 (43/193) | 16.7 (32/192) | 26.0 (44/169) | 0.03 |
| 1,793,445 | T | G | Rv1592c | Glu99Ala | 17 | 0.32 | 6.2 (7/112) | 6.0 (7/117) | 20.0 (23/115) | 0.002 |
| 1,847,247 | G | C | Rv1639c | silent Thr404 | 9871 | - | 13.3 (15/113) | 17.2 (25/145) | 19.8 (23/116) | 0.2 |
* PAM1 (Point Accepted Mutation 1) gives the probability (multiplied with 10,000) for the particular aa exchange to occur, given that 1% of the aa are changed. ** Amino acid changes with SIFT p probabilities < 0.05 are predicted to affect protein function, based on search in UniProt-SwissProt + TrEMBL 2010_09 databases. *** low confidence prediction.