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Comparison of reactive oxygen species (ROS) in the hulls of brown hull1 (bh1) and Wild type (WT). (a) Determination of malondialdehyde, (b) hydrogen peroxide, (c) catalase (d) superoxide dismutase contents in hulls of WT and bh1 (e) TUNEL assay showing DNA fragmentation in hulls of WT and bh1. Propidium iodide (PI) produces green fluorescence in the tissues of hulls, while a merged view (blue and green fluorescence) displays the positive signals of TUNEL.
