Transgenic assays of complementation and knockout to validate the function of Β-glucosidase (Os1βGlu4). (a) Plant morphology, (b) panicle morphology of Nipponbare (Nip), KONip-1, KO-2Nip, KO-3Nip (c) plant morphology of (d) panicle morphology of WT, bh1, KOWT#1, KOWT#2, complementation line-1, complementation line-2 (left to right, respectively), (e) image showing grain length, (f) grain width of Nip, KONip-1, KO-2Nip, KO-3Nip (from top to bottom), (g) image showing grain length, (h) grain width of WT, KOWT#1, KOWT#2, KOWT#3 (from top to bottom), (i) Graph showing the comparison of seed setting rate of WT, KOWT, Complementation, Nip, KONip, (j) Comparison of agronomic traits of WT, Complementation, bh1, KO line, where NT: Normal temperature (top), HT: High temperature (bottom), GL: Grain length, GW: Grain width, PH: Plant height, TGW: Thousand-grain weight, SSR: Seed setting rate, NFP: Number of filled grains per panicle.