Table 4.

Hydrolysis conditions for preparation of protein hydrolysates from soybean.

Hydrolysis Conditions	Units	Symbol	Proteases
			Alcalase	Protamex
Incubation temperature	°C	T	50	55
pH	pH	pH	8.0	7
Enzyme/Substrate ratio (w/w)	%	E/S	1, 1.5, 2, 2.5, 3, 3.5	1, 1.5, 2, 2.5, 3, 3.5
Soy protein/buffer ratio	g/mL	S/L	1:1, 1:2, 1:3,1:4, 1:5	1:1, 1:2, 1:3,1:4, 1:5
Incubation time	h	H	1, 3, 5, 7, 8	1, 3, 5, 7, 8
Inactivation temperature	°C	T	90	90
Inactivation time	min	-	20	20