TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Relevant characteristic(s)a | Source or reference |
|---|---|---|
| E. coli | ||
| DH5αphe | F− φ80dlacZ Δ(lacZYA−argF)U169 recA1 endA1 hsdR17 | 18, 22 |
| HB101 | F− Δ(gpt-proA)62 leuB6 supE44 ara-14 galK2 lacY1 | 16 |
| R. sphaeroides | ||
| 2.4.1 | Wild type | W. Sistrom |
| JZ724 | har-3 | 53 |
| Plasmids | ||
| pBSIISK+ | Apr | Stratagene |
| pBBRMCS2 | Knr | 26 |
| pCF1010 | Derivative of RSF1010; Tcr Spr/Str; used for creating lacZ transcriptional fusions | 30 |
| pJZ20 | 9.5-kb BamHI fragment from R. sphaeroides JZ724 in pUI1087 | This study |
| pLE20 | 4.5-kb ClaI fragment from P. denitrificans in pBSIISK+ | 5 |
| pLF14 | 11.5- and 3.3-kb SalI fragments from R. sphaeroides JZ724 in pUI1087 | This study |
| pLF15 | Amplified hbdA sequences ligated into MscI-treated pUI1087 | This study |
| pLF16 | XbaI-EcoRI fragment containing hbdA from pLF15 in pBBRMCS2 | This study |
| pRK2013 | ColE1 replicon; Tra+ of RK2; Knr | 16 |
| pUI1087 | pBSIISK+ with modified polylinker | 54 |
| pUI1088 | ΩSpr/Str-orfA2-hemA::lacZYA′ in RSF1010 | 52 |
a
Apr, ampicillin resistant; Knr, Kanamycin resistant; Spr/Str, spectinomycin resistant and streptomycin resistant.