Figure 3.

SMKI-specific effects on viral entry. A549 cells were pretreated with SMKIs for 2 h and then infected with either the NL09 or NL11 strain (MOI = 10) for 30 min +/− SMKIs [1×]_max. Cells were fixed and permeabilized, and then virions were detected by anti-NP (green) antibody, F-Actin was detected by ActinRed-555 (red) and nuclei were detected using NucBlueLive ReadyProbes (blue). Virion localization was visualized by confocal microscopy (representative images from two independent experiments).