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SMKI inhibition of IAV is maintained over serial passaging. The stability of SMKI treatment on NL09 and NL11 replication was determined by five serial passages on MDCK cells infected at MOI = 0.001 for 72 h in the presence of the [1×]max SMKI concentrations (n = 4) at each passage. At each passage, viral titers were quantified to inoculate the next passage at the same MOI; means ± SDs are shown. l.o.d.: limit of detection.
