FIG. 5.

Absorption spectra of adenosyltransferase assay mixtures. The spectra indicate the predominant corrinoid present in the assay mixture: prior to the addition of titanium(III) citrate, the spectrum is that of HOCbl (–·–·); after the addition of titanium(III) citrate, the spectrum is that of cob(I)alamin (…………); 10 min after the addition of an inclusion body preparation of the PduO enzyme, the spectrum is that of AdoCbl (–––); after photolysis, the spectrum is that of cob(I)alamin (——).