TABLE 1.
Bacterial strains
| Species and strain | Genotype |
|---|---|
| E. coli | |
| DH5α | F−λ−endA1 hsdR17 relA1 supE44 thi-1 recA1 gyrA96 relA1 Δ(lacZYA-argF)U169 (φ80dlacZΔM15) |
| BL21DE3 RIL | BF−ompT hsdS (rB− mB−) dcm+ Tetrgal λ(DE3) endA Hte[argU ileY leuW Camr] |
| S17.1 λ pir | recA (RP4-2-Tc::Mu)λ pir |
| BE11 | (E. coli ER2267) F− e14− (McrA−) endA1 supE44 thi-1 relA? rfbD1? spoT? Δ(mcrC-mrr)114::IS10 Δ(argF-lac)U169 recA1/F′ ProA+B+lacIq Δ(lacZ)M15 zzf::mini-Tn10(Kanr)/pMGS2 |
| BE115 | DH5α/pTA925 |
| BE116-BE118 | BL21DE3 RIL/pTA926 (T7 expression vector with pduO insert) |
| BE119 | BL21DE3 RIL/pTA925 (T7 expression vector without insert) |
| S. enterica serovar Typhimurium LT2 | |
| TR6579 | metA22 metE551 trpD2 ilv-452 hsdLT6 hsdSA29 HsdB−strA120 GalE− Leu− Pro− |
| BE47 | thr-480::Tn10dCam |
| BE83 | cobA366::Tn10dCam |
| BE111 | ΔpduO651 |
| BE112 | metE205 ara-9 Δ299/pDP1 (entire cob and pdu operons are deleted, and plasmid contains pduO under plac control) |
| BE113 | metE205 ara-9 Δ299/placIqPOBglII |
| BE114 | ΔpduO651 cobA366::Tn10dCam/pDP1 (pduO under lacp control) |
| BE121 | ΔpduO651 cobA366::Tn10dCam |
| BE122 | LT2/pDP1 (pduO under plac control) |
| BE129 | ΔpduO651 cobA366::Tn10dCam/placIqPOBglII |