Figure 4.

Biodistribution of 1% rhodamine-loaded nanoparticles in L. vannamei post-RG administration. Shrimp were injected with 250 μg of either 20:80 CPTEG:CPH or 20:80 CPH:SA rhodamine-loaded nanoparticles by reverse gavage (RG). At indicated time intervals, animals were dissected into cephalothorax, hepatopancreas, gut, gills, and stomach. (A) Qualitative image analysis. Tissue samples were placed on an imaging tray and rhodamine fluorescence was captured using Carestream Multispectral Imager. Images shown are representative of results obtained from two independent experiments. (B) In vivo fluorescence quantification of rhodamine. Data are expressed as the mean fold change over controls ± SEM of two independent experiments. Statistical differences (p < 0.05) of either nanoparticle treatment in comparison to the control are indicated by *, while differences between the two nanoparticle formulations are indicated by #.