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. 2022 Aug 30;10(9):1428. doi: 10.3390/vaccines10091428

Figure 5.

Figure 5

Shrimp survival against WSSV challenge. (A) Shrimp were injected by reverse gavage (RG) with 11 μg of either soluble WSSV 477 dsRNA or 11 μg WSSV 477 dsRNA encapsulated in 100 μg 20:80 CPTEG:CPH or 20:80 CPH:SA nanoparticles. Three days postvaccination animals were challenged with a lethal dose of WSSV and survival was monitored through day 12. Sham controls were injected with water prior to challenge. Soluble eGFP was used as non-shrimp specific vaccination control. The negative controls did not receive any viral dose. n = 60 (two independent experiments with 30 animals/treatment) except the negative control group (n = 40). (B) Photomicrographs of shrimp tissue postchallenge. Scale bar = 20 μm. (C) Viral load measured using WSSV-specific qRT-PCR.