Skip to main content
. 2022 Sep 18;23(18):10932. doi: 10.3390/ijms231810932

Figure 1.

Figure 1

Effects of 2-arachidonoyl LPI on cell rounding of GPR55-expressing HEK293 cells. (A) Morphological changes induced by 2-arachidonoyl LPI. Vector-transfected cells (left) or GPR55-expressing cells (right) were challenged with 2-arachidonoyl LPI (1 µM) at 37 °C for 0 or 5 min. Scale bar, 10 μm. The results are representative of three independent experiments, which gave similar results. (B) Time course of cell rounding. Vector-transfected cells (left) or GPR55-expressing cells (right) were treated with the vehicle (DMSO; open circles) or 2-arachidonoyl LPI (1 µM; closed circles) at 37 °C for the indicated periods. (C) Dose-dependent effects of LPI on cell rounding. Vector-transfected cells (left) or GPR55-expressing cells (right) were treated with the indicated concentrations of 1-stearoyl LPI (closed circles) or 2-arachidonoyl LPI (closed circles) at 37 °C for 5 min. (D) Effects of various cannabinoids and related compounds. Vector-transfected cells (left) or GPR55-expressing cells (right) were treated with the indicated compounds (1 µM) at 37 °C for 5 min. After treatment, images of cells were obtained, and cell rounding was analyzed. Statistical analyses were performed with one-way analysis of variance (ANOVA), followed by Dunnett’s multiple comparison test (B) or Tukey–Kramer multiple comparison test (C,D). The values are means ± SDs of five determinations. * p < 0.05, *** p < 0.001 versus values at 0 min (B) and versus control values obtained with 0 nM LPI (C,D).