Figure 2.

Effects of various species of LPI and related molecules on cell morphology in HEK293 cells stably expressing GPR55. Vector-transfected cells (open circle) or GPR55-expressing cells (closed circle) were challenged with various concentrations of 1-oleoyl LPI (A), 2-oleoyl LPI (B), 1-linoleoyl LPI (C), 2-linoleoyl LPI (D), 1-palmitoyl LPI (E), AM251 (F), SR141716A (G), or 1-stearoyl LPGlu (H) at 37 °C for 5 min. After treatment, images of cells were obtained, and cell rounding was analyzed. Statistical analyses were performed with one-way analysis of variance (ANOVA), followed by Dunnett’s multiple comparison test. The values are means ± SDs of five determinations. * p < 0.05, ** p < 0.01, and *** p < 0.001 versus values with vehicle alone (n = 5).