Figure 4.

Effects of various inhibitors on the 2-arachidonoyl LPI-induced activation of ERK or increases in intracellular Ca²⁺ in GPR55-expressing cells. The 2-Arachidonoyl LPI (1 µM)-induced phosphorylation of ERK (A) or increases in intracellular Ca²⁺ (B) were measured after GPR55-expressing cells were pretreated with the indicated inhibitors. Statistical analyses were performed with one-way analysis of variance (ANOVA), followed by Tukey–Kramer multiple comparison test. The values are means ± SDs of four determinations. * p < 0.05 and *** p < 0.001 versus 2-arachidonoyl LPI alone.