Table 1.
The purity and the concomitants of soybean- and liver-derived LPIs. These LPIs were prepared by treating soybean or bovine liver PI with Naja atra PLA2 or Rhizopus delemar lipase under the indicated pH conditions. Values represent the means ± SDs from three independent experiments.
| 1-16:0 LPI # (% ±SD) |
1-18:2 LPI * (% ±SD) |
2-18:2 LPI * (% ±SD) |
1-18:0 LPI # (% ±SD) |
2-20:4 LPI # (% ±SD) |
||
|---|---|---|---|---|---|---|
| Parent PI | Soybean PI | Soybean PI | Soybean PI | Liver PI | Liver PI | |
| Fatty Acid at sn-1 or -2 |
Enzyme, pH |
PLA2, 7.4 |
Lipase, 8.8 $ |
Lipase, 5.6 |
PLA2, 7.4 |
Lipase, 5.6 |
| 1-Palmitoyl (16:0) | 67.4 ± 0.66 | 0.49 ± 0.22 | 0.08 ± 0.02 | 8.55 ± 0.12 | 0.00 ± 0.00 | |
| 2-Palmitoyl (16:0) | 4.06 ± 0.15 | 0.01 ± 0.01 | 0.21 ± 0.03 | 0.59 ± 0.02 | 0.10 ± 0.04 | |
| 1-Stearoyl (18:0) | 14.2 ± 0.83 | 0.10 ± 0.14 | 0.18 ± 0.26 | 82.42 ± 0.38 | 0.21 ± 0.05 | |
| 2-Stearoyl (18:0) | 0.71 ± 0.09 | 0.00 ± 0.00 | 0.01 ± 0.02 | 5.71 ± 0.14 | 0.00 ± 0.00 | |
| 1-Oleoyl (18:1) | 5.02 ± 0.23 | 8.14 ± 0.18 | 2.76 ± 0.28 | 2.51 ± 0.10 | 0.56 ± 0.07 | |
| 2-Oleoyl (18:1) | 0.18 ± 0.04 | 0.16 ± 0.23 | 7.99 ± 0.58 | 0.09 ± 0.01 | 5.21 ± 0.29 | |
| 1-Linoleoyl (18:2) | 7.34 ± 0.13 | 78.73 ± 0.28 | 22.31 ± 2.56 | 0.07 ± 0.01 | 0.90 ± 0.07 | |
| 2- Linoleoyl (18:2) | 0.32 ± 0.02 | 5.40 ± 0.12 | 57.37 ± 2.47 | 0.00 ± 0.00 | 10.08 ± 0.51 | |
| 1-Linolenoyl (18:3) | 0.70 ± 0.09 | 6.68 ± 0.29 | 2.59 ± 0.38 | 0.00 ± 0.00 | 0.00 ± 0.00 | |
| 2-Linolenoyl (18:3) | 0.01 ± 0.00 | 0.29 ± 0.08 | 6.49 ± 0.27 | 0.00 ± 0.00 | 0.02 ± 0.01 | |
| 1-Arachidonoyl (20:4) | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.06 ± 0.01 | 8.48 ± 0.47 | |
| 2-Arachidonoyl (20:4) | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | 74.43 ± 0.45 | |
* LPI was used in this study. # LPI was not used in this study. $ The treatment with lipase at pH 8.8 facilitated the fatty acid migration from the sn-2 to sn-1 positions of glycerol to form 1-acyl LPI.