Figure 5.

(A) ELISA determination of PGE₂ released from A549 cells into culture media. (B) Representative immunochemical analysis of COX-2 and AGR2. Beta-actin served as a loading control and for normalization to determine fold changes calculated by densitometric analysis in cells exposed to prostaglandin E₂ (PGE₂), diclofenac (DIC), celecoxib (CXB), and acetylsalicylic acid (ASA) compared to untreated cells (CTR). Significant changes (p < 0.05) determined from three independent biological experiments are indicated by asterisk.