TABLE 3.
Transfer frequencies for pVT745 derivatives and pMMB67 from an A. actinomycetemcomitans donor strain into different recipients
| Donor strain | Donor plasmid(s) | No. of transconjugants per donora in recipient strain(s):
|
||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| ATCC 29522Rif and ATCC 29522::pDMG3
|
ATCC 700685:pDMG3:
|
JM109
|
||||||||
| KAN | AMP | KAN AMP | KAN | AMP | KAN AMP | KAN | AMP | KAN AMP | ||
| Conjugation | ||||||||||
| JP2 | pDMG20 | 10−5 | NA | NA | 10−5 | NA | NA | 10−6 | NA | NA |
| JP2 | pDMG21 | 10−5 | NA | NA | 10−4 | NA | NA | <10−9 | NA | NA |
| Mobilization | ||||||||||
| JP2 | pDMG20, pMMB67 | 10−6 | 10−5 | 10−6 | NA | NA | NA | 10−5 | 10−6 | 10−7 |
| JP2 | pDMG21, pMMB67 | 10−6 | 10−6 | 10−6 | NA | NA | NA | <10−9 | 10−5 | <10−9 |
a
The number of transconjugants per donor (average of at least two independent experiments) is shown. The selection of transconjugants was done for the recipient strain as indicated in the text and with kanamycin (KAN), ampicillin (AMP), or both for the incoming plasmid(s) as shown in the table. The ATCC strains are A. actinomycetemcomitans; strain JM109 is E. coli. NA, not applicable.