Figure 3.
Therapeutic vaccination is associated with clearance of existing ovarian tumors. (A) Longitudinal 2-and 3-dimensional bioluminescence imaging of FVB mice with BR5-Akt-Luc2 ovarian cancer over time with false (simulated) skeletal and vascular systems shown in the final images. (B) Bioluminescent images and graph of tumor burden (photons/s) from FVB mice administered 2 × 105 BR5-akt-Luc2 cells on Day 0 and treatment with 3 × 106 BR5-akt vaccine cells on Days 4 and 11 (n = 3/group; Holm–Sidak multiple comparisons). (C) Animal weight across time in untreated (no Tx) tumor-bearing mice. (D) Flow cytometry was used to define changes in peritoneal effector memory T cell populations following vaccination (Day 25; unpaired, two-tailed, parametric t-test, and SD error bars). * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001.