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. 2022 Sep 10;10(3):51. doi: 10.3390/medsci10030051

Figure 1.

Figure 1

Effect of polyamine modulation of mTORC1 downstream proteins phosphorylation. MDA-MB-231 cells were grown in 5 mM DFMO containing growth medium with no amino acids supplementation for 48 h. Cells were thereafter serum starved for 12 h after which medium was refreshed and supplemented with 100 µM and 50 µM spermidine and spermine, respectively, for the indicated time. Thereafter cells were harvested and processed for Western blot protein expression determination. (a) Level of phosphorylated 4EBP1 and p70S6K as well as the level of eIF4E were determined on 12% SDS PAGE. β-actin was used as a loading control, Ctrl represent untreated control (b) Gel densitometry tracing from (a) was estimated using ImageJ software. Values shown are mean ± range, for two independent experiments for 4EBP1 and p70S6K.