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. 2022 Sep 10;10(3):51. doi: 10.3390/medsci10030051

Figure 5.

Figure 5

Effect of DFMO and rapamycin on viability of breast cancer cells. Cells were treated with DFMO and rapamycin, singularly and in combination at the indicated concentrations for 48 h, cytotoxicity was measured using MTT assay. (a) MDA-MB-231 cells in the presence of DFMO and rapamycin. (b) MCF-7 cells in the presence of DFMO and rapamycin (because MCF-7 cell was more sensitive to rapamycin treatment, lower concentrations was used). The results were expressed as the percentage of viable cells compared to the untreated control. Values shown are mean ± S.E.M, of at least 18 wells from three independent experiments for MCF-7 and mean ± range, of at least 12 wells from two independent experiments for MDA-MB-231. Note: Rapa represents rapamycin. Statistics was performed using one-way ANOVA with Dunnett’s post hoc analysis, where *** p < 0.001 vs. DFMO alone. The values above the bars for the combination treatment are the EOB scores.