Table 3.

Cannabinoids content (%) in tinctures (20% ethanol, 40% ethanol, and 80% ethanol) prepared from inflorescences of the Futura 75, KC Dora, and Tygra varieties (HPLC method).

Extraction Method (100 rpm, 72 h)	Variety	Plant Material	Total CBD (%)	Total Δ9-THC (%)
20% EtOH	Futura 75	F/10/90	0.081 ± 0.009 a	0.000 ± 0.000 a
		F/30/30	0.071 ± 0.005 a	0.000 ± 0.000 a
	KC Dora	K/30/30	0.072 ± 0.005 a	0.000 ± 0.000 a
		K/30/90	0.064 ± 0.004 a	0.000 ± 0.000 a
	Tygra	T/10/30	0.070 ± 0.006 a	0.000 ± 0.000 a
		T/30/30	0.063 ± 0.011 a	0.000 ± 0.000 a
40% EtOH	Futura 75	F/10/90	0.581 ± 0.093 b	0.004 ± 0.003 a
		F/30/30	0.616 ± 0.041 b	0.006 ± 0.002 ab
	KC Dora	K/30/30	0.619 ± 0.021 b	0.001 ± 0.000 a
		K/30/90	0.581 ± 0.005 b	0.000 ± 0.000 a
	Tygra	T/10/30	0.535 ± 0.037 b	0.002 ± 0.000 a
		T/30/30	0.557 ± 0.021 b	0.003 ± 0.001 a
80% EtOH	Futura 75	F/10/90	1.305 ± 0.000 ef	0.045 ± 0.020 bc
		F/30/30	1.393 ± 0.005 f	0.048 ± 0.026 c
	KC Dora	K/30/30	1.262 ± 0.037 ef	0.057 ± 0.002 c
		K/30/90	1.169 ± 0.022 de	0.026 ± 0.000 abc
	Tygra	T/10/30	0.997 ± 0.040 cd	0.045 ± 0.007 bc
		T/30/30	0.952 ± 0.160 c	0.036 ± 0.025 abc

CBD–cannabidiol, Δ⁹-THC–Δ⁹-tetrahydrocannabinol. ^a–f Mean values with a column with the same letter are not significantly different at α = 0.05 (Tukey’s test).