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. 2022 Sep 6;27(18):5771. doi: 10.3390/molecules27185771

Figure 4.

Figure 4

Comparison of holdase chaperone activity between AtPFN1 and AtPFN2. Thermal aggregation of 20 µg malate dehydrogenase (MDH) was examined at 43 °C for 20 min in the presence of AtPFN1 or AtPFN2 proteins. (a) Molar ratio of AtPFN2 to MDH: () 3:1, () 5:1, and (●) 10:1. (◆) denotes the negative Control (MDH alone). (b) Molar ratios of AtPFN1 to MDH: () 5:1, () 20:1, and (●) 40:1. (◆) denotes the negative control (MDH alone). Holdase chaperone activity by measuring the absorbance of the solutions at a wavelength of 340 nm.