Schematic of the regulatable pilE gene of C9.10. Shaded boxes indicate genes and are drawn to scale. Arrows extended from the gene names indicate the direction of transcription. Thick arrows indicate the operators and promoters. P, pilE2 promoter. Restriction sites are abbreviated in capital letters as follows: B, Bsu36I; C, ClaI, H, HpaI, N, NotI, S, SmaI. Crossed-out restriction sites were destroyed during construction.