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. 2022 Sep 15;23(18):10761. doi: 10.3390/ijms231810761

Figure 5.

Figure 5

Log relative fold-change in mRNA expression by qRT-PCR of genes involved in biofilm formation for planktonic initial, planktonic biofilm-detached (PBD, blue)) and sessile (S, yellow) S. aureus and P. aeruginosa strains cultivated 72-h alone in CWM inside the open microfluidic system BioFluxTM 200. Log relative fold change in expression was determined for fnbpA, spaA (A), hla, agrA (B) in S. aureus and pel, psl (C), rhII, lasI, (D) in P. aeruginosa. Reference strains are represented by solid colors, the two clinical strains are represented in squared and hatched patterns. Samples were tested in triplicate in two independent experiments. Results are expressed as the mean fold change (planktonic initial cells representing the control standardized and the reference) with error bars representing the standard deviation. Statistics were performed using one-way ANOVA followed by Dunnett’s multiple comparisons test on GraphPad Prism version 9.2. * p < 0.1; ** p < 0.01.