Figure 1.

NMDA-induced Ca²⁺ influx involves GluN2B but not GluN2A, -2C, or -2D complexes in cerebrocortical neurons. Pharmacologic evidence indicating that NMDA-induced Ca²⁺ influx involves GluN2B but not GluN2A or -2C/2D complexes in cerebrocortical neurons via interrogation with NMDA channel blocker MK-801 (A), non-competitive GluN2A antagonist TC-201 (B), non-competitive GluN2B antagonist ifenprodil (C), and GluN2C and 2D antagonist QNZ-46 (D). Genetic evidence confirming that GluN2C and GluN2D NMDAR subtypes are not required for NMDA-induced Ca²⁺ influx in cerebrocortical neurons in control C57BL/6 (E), GluN2C-KO (F), and GluN2D-KO (G) in DIV 10–12 cerebrocortical neurons. This experiment was repeated in triplicate. Data shown represent the mean ± SEM of 3 experiments.