Table 1.

The susceptibility of planktonic- and biofilm-forming cells of F. pedrosoi and P. verrucosa to antifungal agents.

	F. pedrosoi MIC (µM/mg/L)			P. verrucosa MIC (µM/mg/L)
Antifungal Agents	Planktonic	Biofilm	bMIC/MIC	Planktonic	Biofilm	bMIC/MIC
Amphotericin B	6.25/5.78	200/185	↑ 32×	3.12/2.88	25/23	↑ 8×
Ketoconazole	0.39/0.21	800/425	↑ 2051×	50/27	400/213	↑ 8×
Itraconazole	0.19/0.13	>800/>560	↑ >4200×	0.19/0.13	>800/>560	↑ >4200×
Posaconazole	0.04/0.03	>800/>600	↑ >20.000×	0.01/0.007	400/280	↑ 40.000×
Terbinafine	0.78/0.23	>800/>230	↑ >1000×	0.04/0.01	>800/>230	↑ >20.000×

Planktonic cells (1 × 10⁴) and mature biofilm (initial inoculum of 1 × 10⁴) were treated with different antifungal agents. The minimum inhibitory concentration (MIC) was defined as 100% growth inhibition using visual inspection as recommended by CLSI [56] and confirmed with XTT colorimetric assay [24]. The calculation considered the minimum drug concentration not able to promote the XTT reduction, representing cells metabolically inactivate and/or nonviable. The MIC was expressed in both micromolars (µM) and milligrams per liter (mg/L). (↑) Represents the order of magnitude, in which biofilm MIC (bMIC) was higher than the MIC of planktonic cells. Biofilm-forming cells of F. pedrosoi and P. verrucosa showed significantly higher MICs (p < 0.05) than planktonic cells for all tested antifungal agents.