Figure 5.

HPLC analysis of the reaction catalyzed by CsD27-1 in vivo on β-carotene produced by E. coli cells. (a) pThio+CsD27-1, converts all-trans-β-carotene (III) to 9-cis-β-carotene (IV), 13-cis-β-carotene (II) and to15-cis-β-carotene (I). (b) Percentages of all the cis-β-carotene isomers were significantly increased in the cells expressing the CsD27-1 enzyme. Statistical analysis was performed using student-t test. A designation of ** = p < 0.01 (t-test). n = 3 independent biological replicate experiments. Error bars represent SD.