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. 2022 Sep 11;23(18):10543. doi: 10.3390/ijms231810543

Figure 6.

Figure 6

In planta activity of CsD27-1. (a) HPLC analysis of in vivo CsD27-1 activity in leaves of N. benthamiana plants grown during 48 h in dark conditions. (b) HPLC analysis of in vivo CsD27-1 activity in leaves of N. benthamiana plants grown under standard light conditions. (c) Percentage of cis-9-β-carotene isomer was significantly increased in N. benthamiana leaves agroinfiltrated with 35S::CsD27-1 in both dark and under standard light conditions. Ratio was calculated based on peak area of 9-cis-β-carotene to all-trans-β-carotene in the chromatograms in (a,b). (d) Increase in apocarotenoid levels in N. benthamiana leaves agroinfiltrated with 35S::CsD27-1 in both dark and under standard light conditions. Statistical analysis was performed using the Student t-test. Asterisks indicate differences from wild type (** = p < 0.01, *** = p < 0.001 t-test). n = 3 independent biological replicate experiments. Error bars represent SD.