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. 2022 Sep 11;23(18):10543. doi: 10.3390/ijms231810543

Figure 7.

Figure 7

Analysis of CsD27-3 activity in E. coli cells accumulating the linear carotenoids ζ-carotene and neurosporene. (a) HPLC analyses of carotenes extracted in the absence (pThio-θ) and presence (pThio+CsD27-3) of CsD27-3. MaxPlot chromatograms showing each peak at its spectra are presented. Peak I: 9,15,9′-tri-cis-ζ-carotene; peak II: 9,9′-di-cis-ζ-carotene; peak III: all-trans-ζ-carotene. Absorption spectra of specific peaks are presented in inset boxes. 9,15,9′-tri-cis-ζ-carotene is distinguished from the 9,9′-cis-isomer by the typical absorbance at 296 nm. (b) Percentage of 9,15,9′-tri-cis-ζ-carotene isomer was significantly increased in the cells expressing the CsD27-3 enzyme. A designation of ** = p < 0.01 (t-test). n = 3 independent biological replicate experiments. Error bars represent SD. (c) HPLC analyses of carotenes extracted in the absence (pThio-θ) and presence (pThio+CsD27-3) of CsD27-3. MaxPlot chromatograms showing each peak at its spectra are presented. Peak I: 15-cis-neurosporene; peak II: 13-cis-neurosporene; peak III: 9-cis-neurosporene, and peak IV: all-trans-neurosporene. Absorption spectra of specific peaks are presented in boxes. (d) Percentage of all cis-isomers was significantly increased in the cells expressing the CsD27-3 enzyme. Statistical analysis was performed using the Student t-test. A designation of *** = p < 0.001 (t-test). n = 3 independent biological replicate experiments. Error bars represent SD.