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Confirmation of the overexpression of N-terminal/central part of EFVeca Gag protein; (A) SDS-PAGE with the E.coli lysate before (t0) and five hours after induction with IPTG (t5) and purified Gag protein; (B) detection of EFVeca Gag antigen by immunoblotting with horse serum samples from PCR EFVeca positive and negative individuals. The band of approximately 42 kDa corresponds to the recombinant EFVeca Gag antigen fused with His-tag.
