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. 2022 Sep 10;23(18):10492. doi: 10.3390/ijms231810492

Figure 2.

Figure 2

Multiple-generational inheritance of the epi-edited Krot 0-65dc. (a) Chop-PCR analysis of plants from T2 and T3 generations. The red numbers indicate the lines used in the experiment; (b) T4 generation without CRISPR/dCas9-TET1cd vector, the left is the results of T4 generation detection Chop-PCR and without dCas9, and the right is the T4 generation detection without TET1cd and sgRNAs. (c) PPH gene expression in Per 1, C24, Krot 0 WT (Krot 0 wild type), T2, and T4 generations (n = 3, *, p < 0.05, t-test).