TABLE 2.
Primers
| Primer | Sequencea | Enzymeb | Positionc |
|---|---|---|---|
| OL12 | AAAATTCTCCAGTCTTCACATC | NAd | −117 |
| OL13 | CGACTAAGAAAATGCCGTC | NA | +5226 |
| OL81 | TTTTAAGCTTCCCGGGACTGAAATC | HindIII | +745 |
| OL82 | TTTTTGGATCCAAAGAGGTCTACCGG | BamHI | +2645 |
| OL85 | AAAAACTCGAGATGCATCACTGCAGA | XhoI | +1 |
| OL86 | AAAAAGGATCCTTAGTAGTCGCAGCAAGG | BamHI | +210 |
a
The restriction endonuclease site in each oligonucleotide is underlined.
b
Enzyme that cuts the sequence.
c
Position bound by the first nucleotide of each primer. The translational start site of cotD and the first nucleotide of the amyE open reading frame in pFC1 are designated +1.
d
NA, not applicable.