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. 2022 Sep 8;14(9):1905. doi: 10.3390/pharmaceutics14091905

Figure 4.

Figure 4

Effect of AS-EVs on the proliferation and migration of HDFs. (A) After serum starvation, HDFs were treated with AS-EVs for 48 h in serum-free DMEM/F12. The population of viable cells was evaluated by WST-8 assay. Statistical significance was determined by comparison with the control group not treated with AS-EVs if not indicated (** p < 0.01, *** p < 0.005, n = 4). (B) Migration of HDFs treated with AS-EVs was evaluated by transwell migration assay. After 24 h treatment with AS-EVs, the HDFs that had migrated to the bottom side of the porous membrane were stained with CV and followed by fixation with 4% PFA. Scale bar indicates 200 μm. (C) For quantitative analysis, the stained membrane was cut out and immersed in a 50% acetic acid solution to dissolve the crystalized CV. The intensity of CV staining was determined as optical absorbance at 560 nm. Statistical significance was determined by comparison with the control group not treated with AS-EVs (*** p < 0.005, n = 3).