FIG. 5.

Export of the hook-capping protein FlgD to the periplasm (p) and culture supernatant (s) of cells of wild-type (wt) SJW1103, the first-site fliF mutant SJW2706 (fliF∗), the pseudorevertant (fliF∗ flhA∗), and the flgD null mutant SJW156 (flgD). Samples were subjected to SDS-PAGE and immunoblotted with polyclonal anti-FlgD antibody. Size in kilodaltons is indicated at the left.