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Titration of inoculum in the presence or absence of anti-MV antibody. (A) Back titration of the inoculum was performed by either pipetting or expunging the inoculum through a syringe subsequent to plaque assay. Antibody known to neutralize the MV form of monkeypox virus (7D11) was used to evaluate the morphogenic content (EV and MV). (B) The inoculums subsequently underwent four sequences of a freeze/thaw and three sonication cycles, after which a neutralization assay was performed.
