Figure 6.

Effect of serum on monkeypox virus comet formation. (A,B) Two concentrations of monkeypox virus were adsorbed on Vero E6 cells for 2 h and unattached virus was subsequently removed by washing. A final concentration of 10% cynomolgus serum or heat-inactivated serum in the presence or absence of anti-L1 antibody (1:500) was used to overlay the cells and a 1.5% methylcellulose overlay with and without anti-L1 antibody was utilized as a no-comet control and to determine the effect the antibody on the primary plaques. (C) Percent primary plaque reduction in the presence of anti-L1 antibody within each matrix listed on the X axis. (D) Percent primary plaque reduction of serum using either the heat-inactivated serum or media to normalize. High virus concentration was a target of 100 pfu/well, whereas intermediate virus concentration was a target of 50 pfu/well.