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. 2022 Sep 14;14(9):1949. doi: 10.3390/pharmaceutics14091949

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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The total expressed reporter levels in adherent cells do not correlate with therapeutic protein production efficacy. (a) Transfection efficacy is assessed by quantitation of luminescence from cell lysate 24 h post-transfection of CHO-K1 adherent cells with pLuc. The top performers are highlighted in green. N = 5. (b) The production of Trastuzumab mAb in CHO 1H7 suspension cell culture in serum-free defined media 7 days after the transient transfection of pDNA. The complexes with PEI were formed at N/P ratio 20, and the complexes with CPPs were formed at CPP/pDNA charge ratio 2.5:1. They were assessed by SDS-PAAG 10%, with Coomassie blue staining. (c) List of reagents included in the further study. (d) A scheme depicting general workflow.