Figure 1.
High-Resolution 1H NMR Analysis of Human Salivary Supernatant Samples. (a,b), High- and low-field regions (0.70–4.40 and 6.75–9.65 ppm, respectively) of the 600 MHz noesy-presat 1H NMR spectrum of a WMSS sample donated by a healthy human participant. Samples were prepared according to the methods described in Refs. [1,2], and contained added phosphate buffer to control pH, azide as a microbicide to curtail metabolite consumption/fermentation and catabolite generation by bacteria during periods of sample preparation and storage prior to analysis, and 250 μmol./L TSP as a chemical shift reference (δ = 0.00 ppm) and quantitative 1H NMR standard. (c,d), 1.60–2.50 ppm regions of repeat noesy-presat and WET pulse sequence 1H NMR profiles acquired on a human WMSS sample for 512 and only 8 scans, respectively. The noesy-presat or WET pulse sequences were employed to suppress the intense water resonance. Typical spectra are shown. Resonance assignment number codes and abbreviations are listed in Table 1. The asterisks in (c,d) represent 13C satellites of the very intense WMSS acetate-CH3 resonance.