Figure 6.

¹H NMR Evaluation of the Treatment of WMSS Samples with the Enzyme Hyaluronidase. Partial (1.50–2.26 ppm regions of) 600 MHz WASTED-II ¹H NMR spectra of (a) an aqueous solution containing a commercially available hyaluronidase enzyme preparation (4167 units/mL in aqueous solution containing 8.33% (v/v) ²2H₂O); (b) a typical untreated WMSS specimen; (c), as (b), but treated with 4167 units/mL of the hyaluronidase preparation (both control and hyaluronidase-treated saliva samples also contained 8.33% (v/v) ²2H₂O). Both untreated and treated saliva samples were equilibrated for a duration of 8.00 h. at pH 6.50 and 25 °C prior to ¹H NMR analysis. Assignment abbreviations: as Table 1. The arrows in (c) indicate resonances arising from ¹H NMR-active contaminants introduced from the added enzyme preparation. Spectra were recorded on a Jeol JNM-ECZ600R/S1 600 MHz spectrometer operating at frequency of 600.17 MHz for ¹H (probe operating temperature 25 °C).