Table 1.
Primer | Sequence * |
---|---|
pln_Aat | GACTGACGTCTTCTAGACGGCTTCGGCAGCGAA |
m4in_Sac | AACTGAGCTCGCTGTCCTCTTCGGCCAAGTTGT |
op_combD | CTATGCCGGATGAGTGAACACTTACA |
op_combR | TTCACTCATCCGGCATAGTGGCTCTCC |
m4in_Aat | GACTGACGTCTAGATTTACTTCCAGCAAGC |
m4in_combD | TGGCATAAACACTTACAACGGCTGATAAC |
m4in_combR | GTTGTAAGTGTTTATGCCACCCCCACCTGAT |
op_exR | GATTATTGGTCTGGTGTGCG |
op_exD | AACATATTCACCAGTTAGCCG |
m4in_exD | AGGCTTTCAAACGCAACTCGC |
op_inR | TTGGGCATCTATTTCTTACG |
op_inD | ACGAACTGGGCTATGAGG |
m4in_inD | ATGAATCGCTGTCTGACG |
* The Aat II sites are underlined, the Xba I sites are boldfaced, the Sac I site is italicized and boldfaced. The overlapping sequences are underlined with a double or wavy line. All primers were obtained from Evrogen (Russia).