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. 2022 Sep 17;20(9):580. doi: 10.3390/md20090580

Figure 1.

Figure 1

Effect of high molecular weight fucoidan (HMWF) on methylglyoxal-derived hydroimidazolone-1 (MG-H1) induced cell cytotoxicity of the human colon carcinoma cell line (Caco-2) cells. (A) Cells were treated with 500 μg/mL HMWF and 10 μM MG-H1 for 24 h. Caco-2 cell viability was evaluated using 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay. (B) Cells were treated with 500 μg/mL HMWF and 10 μM MG-H1 for 24 h. The reactive oxygen species (ROS) level was evaluated by 2,7-dichlorofluorescin diacetate assay. The results are shown as mean ± standard deviation (n = 3). *** p < 0.001 versus the control cells, ## p < 0.01 versus MG-H1 treated cells.