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Effect of HMWF on localization of occludin in Caco-2 cells. Localization of occludin was measured using immunofluorescence in Caco-2 cells. The cells were differentiated for 5 d and then treated with 500 μg/mL HMWF and 10 μM MG-H1 for 24 h. The localization of occludin (green) and nucleus (DAPI, blue) in intercellular was measured using 20× objective by confocal laser scanning microscope with specific antibody; scale bar 20 μm.
